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Nat Methods. 2013 Jun;10(6):553-6. doi: 10.1038/nmeth.2442. Epub 2013 Apr 14.

A simple tool to improve pluripotent stem cell differentiation.

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1
Department of Stem Cell and Regenerative Biology, Harvard Stem Cell Institute, Cambridge, Massachusetts, USA.

Abstract

We describe a method to help overcome restrictions on the differentiation propensities of human pluripotent stem cells. Culturing pluripotent stem cells in dimethylsulfoxide (DMSO) activates the retinoblastoma protein, increases the proportion of cells in the early G1 phase of the cell cycle and, in more than 25 embryonic and induced pluripotent stem cell lines, improves directed differentiation into multiple lineages. DMSO treatment also improves differentiation into terminal cell types in several cell lines.

PMID:
23584186
PMCID:
PMC3694177
DOI:
10.1038/nmeth.2442
[Indexed for MEDLINE]
Free PMC Article
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