Format

Send to

Choose Destination
See comment in PubMed Commons below
Methods. 2013 Nov;64(1):36-42. doi: 10.1016/j.ymeth.2013.04.001. Epub 2013 Apr 11.

Single molecule FRET data analysis procedures for FRET efficiency determination: probing the conformations of nucleic acid structures.

Author information

  • 1Interdisciplinary Nanoscience Center (iNANO), Aarhus University, Gustav Wieds Vej 14, 8000 Aarhus C, Denmark.

Abstract

Single molecule FRET microscopy is an attractive technique for studying structural dynamics and conformational diversity of nucleic acid structures. Some of its strengths are that it can follow structural changes on a fast time scale and identify conformation distributions arising from dynamic or static population heterogeneity. Here, we give a description of the experiment and data analysis procedures of this method and detail what parameters are needed for FRET efficiency calculation. Using single molecule FRET data obtained on G-quadruplex DNA structures that exhibit large conformation diversity, we illustrate that the shape of the FRET distribution changes depending on what parameters are included in the data analysis procedure.

Copyright © 2013 Elsevier Inc. All rights reserved.

KEYWORDS:

Fluorescence resonance energy transfer; G-quadruplex DNA structures; Nucleic acid structures; Single molecule microscopy

[PubMed - indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Write to the Help Desk