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Anal Biochem. 2013 Aug 1;439(1):4-6. doi: 10.1016/j.ab.2013.04.001. Epub 2013 Apr 11.

Label-free cell profiling.

Author information

1
Medical Cell Biophysics Group, MIRA Institute, University of Twente, Enschede, The Netherlands. r.b.m.schasfoort@utwente.nl

Abstract

A surface plasmon resonance (SPR) array imaging method is outlined for label-free cell profiling. Red blood cells (RBCs) were injected into a flow chamber on top of a spotted sensor surface. Spots contained antibodies to various RBC membrane antigens. A typical sensorgram showed an initial response corresponding to cell sedimentation (S) followed by a specific upward response (T) corresponding to specific binding of cells during a critical wash step. The full analysis cycle for RBC profiling was less than 6 min. The sensor surface could be regenerated at least 100 times, allowing the determination of a cell surface antigen profile of RBCs.

PMID:
23583822
DOI:
10.1016/j.ab.2013.04.001
[Indexed for MEDLINE]

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