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Mem Inst Oswaldo Cruz. 2013 Apr;108(2):246-7.

Use of sodC versus ctrA for real-time polymerase chain reaction-based detection of Neisseria meningitidis in sterile body fluids.

Author information

1
Centro de Imunologia, Instituto Adolfo Lutz, São Paulo, SP, Brasil. higa.fabio@yahoo.com.br

Abstract

We evaluated the use of a newly described sodC-based real-time-polymerase chain reaction (RT-PCR) assay for detecting Neisseria meningitidis in normally sterile sites, such as cerebrospinal fluid and serum. The sodC-based RT-PCR assay has an advantage over ctrA for detecting nongroupable N. meningitidis isolates, which are commonly present in asymptomatic pharyngeal carriage. However, in our study, sodC-based RT-PCR was 7.5% less sensitive than ctrA. Given the public health impact of possible false-negative results due to the use of the sodC target gene alone, sodC-based RT-PCR for the diagnosis of meningococcal meningitis should be used with caution.

PMID:
23579808
PMCID:
PMC3970655
[Indexed for MEDLINE]
Free PMC Article

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