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J Korean Med Sci. 2013 Apr;28(4):608-13. doi: 10.3346/jkms.2013.28.4.608. Epub 2013 Mar 27.

Chlorogenic acid decreases retinal vascular hyperpermeability in diabetic rat model.

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Department of Ophthalmology, Seoul National University College of Medicine, Seoul National University Hospital, Seoul, Korea.


To evaluate the effect of chlorogenic acid (CGA), a polyphenol abundant in coffee, on retinal vascular leakage in the rat model of diabetic retinopathy, Sprague-Dawley rats were divided into four groups: controls, streptozotocin-induced diabetic rats, and diabetic rats treated with 10 and 20 mg/kg chlorogenic acid intraperitoneally daily for 14 days, respectively. Blood-retinal barrier (BRB) breakdown was evaluated using FITC-dextran. Vascular endothelial growth factor (VEGF) distribution and expression level was evaluated with immunohistochemistry and Western blot analysis. Expression of tight junction proteins, occludin and claudin-5, and zonula occludens protein, ZO-1 was also evaluated with immunohistochemistry and Western blot analysis. BRB breakdown and increased vascular leakage was found in diabetic rats, with increased VEGF expression and down-regulation of occludin, claudin-5, and ZO-1. CGA treatment effectively preserved the expression of occludin, and decreased VEGF levels, leading to less BRB breakdown and less vascular leakage. CGA may have a preventive role in BRB breakdown in diabetic retinopathy by preserving tight junction protein levels and low VEGF levels.


Blood-Retinal Barrier; Chlorogenic Acid; Diabetes Rat Model; Diabetic Retinopathy; Vascular Endothelial Growth Factor

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