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EMBO Rep. 2013 Jun;14(6):527-33. doi: 10.1038/embor.2013.47. Epub 2013 Apr 12.

Antisense regulation by transposon-derived RNAs in the hyperthermophilic archaeon Sulfolobus solfataricus.

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1
Department of Microbiology, Immunobiology and Genetics, Max F. Perutz Laboratories, University of Vienna, Dr Bohrgasse 9, 1030 Vienna, Austria.

Abstract

We report the first example of antisense RNA regulation in a hyperthermophilic archaeon. In Sulfolobus solfataricus, the transposon-derived paralogous RNAs, RNA-257(1-4), show extended complementarity to the 3' UTR of the 1183 mRNA, encoding a putative phosphate transporter. Phosphate limitation results in decreased RNA-257(1) and increased 1183 mRNA levels. Correspondingly, the 1183 mRNA is faster degraded in vitro upon duplex formation with RNA-257(1). Insertion of the 1183 3' UTR downstream of the lacS gene results in strongly reduced lacS mRNA levels in transformed cells, indicating that antisense regulation can function in trans.

PMID:
23579342
PMCID:
PMC3674436
DOI:
10.1038/embor.2013.47
[Indexed for MEDLINE]
Free PMC Article
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