Abstract
Twelve alkyl analogues (1-12) of the high-affinity serotonin transporter (SERT) inhibitor 6-nitroquipazine (6-NQ) were synthesized and studied using in vitro radioligand competition binding assays to determine their binding affinity (Ki ). The putative antidepressant activity of five of the binders with the highest SERT binding affinities was studied by the forced swim and locomotor activity mouse tests. The three-dimensional (3D) structures of 8 and 9 were determined using NOE NMR technique. Flexible docking of the compounds was undertaken to illustrate the binding of the compounds in the SERT model. Our results showed that several of the 6-NQ analogues are high-affinity SERT inhibitors and indicated that the octyl (8), decyl (10) and dodecyl (12) 6-NQ analogues exhibit moderate antidepressant activity.
© 2013 John Wiley & Sons A/S.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Antidepressive Agents / chemical synthesis*
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Antidepressive Agents / chemistry
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Antidepressive Agents / pharmacology
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Binding Sites
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Male
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Mice
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Molecular Docking Simulation
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Motor Activity / drug effects
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Protein Binding
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Protein Structure, Tertiary
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Quipazine / analogs & derivatives*
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Quipazine / chemical synthesis
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Quipazine / chemistry
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Quipazine / pharmacology
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Receptor, Serotonin, 5-HT1A / chemistry
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Receptor, Serotonin, 5-HT1A / metabolism
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Selective Serotonin Reuptake Inhibitors / chemical synthesis*
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Selective Serotonin Reuptake Inhibitors / chemistry
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Selective Serotonin Reuptake Inhibitors / pharmacology
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Serotonin Plasma Membrane Transport Proteins / chemistry*
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Serotonin Plasma Membrane Transport Proteins / metabolism
Substances
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Antidepressive Agents
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Serotonin Plasma Membrane Transport Proteins
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Serotonin Uptake Inhibitors
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Receptor, Serotonin, 5-HT1A
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Quipazine
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6-nitroquipazine