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Physiol Mol Biol Plants. 2010 Dec;16(4):353-8. doi: 10.1007/s12298-010-0038-2. Epub 2010 Dec 7.

Difference in in vitro response and esculin content in two populations of Taraxacum officinale Weber.

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Department of Botany, Faculty of Science, Jamia Hamdard, New Delhi, 110062 India ; Department of Biotechnology, Faculty of Science, Jamia Hamdard, New Delhi, 110062 India.


In vitro micropropagation has been achieved in medicinally important plant, Taraxacum officinale collected from two different regions, Kashmir (J & K) and Garhwal (Uttarakhand). Leaf segments inoculated on MS supplemented with different combinations of Indole-3-acetic acid (IAA) and Benzyladenine (BA) produced indirect regeneration. For root induction MS fortified with Indole-3-butyric acid (IBA) was used. Taraxacum officinale collected from Garhwal responded two weeks earlier and showed shoot regeneration whereas in Kashmir population only callus proliferation occurred. Esculin content was also higher in the samples from Garhwal. The content was affected by both, the hormone concentration as well as age of the cultures. RAPD of the in vitro raised regenerants confirmed genetic stability.


Different populations; Esculin; Micropropagation; RAPD; Taraxacum officinale

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