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Dongwuxue Yanjiu. 2013 Apr;34(2):121-6. doi: 10.3724/SP.J.1141.2013.02121.

[Analysis of the molecular characteristics and cloning of full-length coding sequence of interleukin-2 in tree shrews].

[Article in Chinese; Abstract available in Chinese from the publisher]

Author information

1
Institute of Medical Biology, the Chinese Academy of Medical Science, China.

Abstract

in English, Chinese

While the tree shrew (Tupaia belangeri chinensis) is an excellent animal model for studying the mechanisms of human diseases, but few studies examine interleukin-2 (IL-2), an important immune factor in disease model evaluation. In this study, a 465 bp of the full-length IL-2 cDNA encoding sequence was cloned from the RNA of tree shrew spleen lymphocytes, which were then cultivated and stimulated with ConA (concanavalin). Clustal W 2.0 was used to compare and analyze the sequence and molecular characteristics, and establish the similarity of the overall structure of IL-2 between tree shrews and other mammals. The homology of the IL-2 nucleotide sequence between tree shrews and humans was 93%, and the amino acid homology was 80%. The phylogenetic tree results, derived through the Neighbour-Joining method using MEGA5.0, indicated a close genetic relationship between tree shrews, Homo sapiens, and Macaca mulatta. The three-dimensional structure analysis showed that the surface charges in most regions of tree shrew IL-2 were similar to between tree shrews and humans; however, the N-glycosylation sites and local structures were different, which may affect antibody binding. These results provide a fundamental basis for the future study of IL-2 monoclonal antibody in tree shrews, thereby improving their utility as a model.

KEYWORDS:

Cloning; Function; Interleukin 2; Structure; Tree shrews

PMID:
23572362
DOI:
10.3724/SP.J.1141.2013.02121
[Indexed for MEDLINE]
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