Format

Send to

Choose Destination
See comment in PubMed Commons below
Asian Pac J Trop Biomed. 2012 Sep;2(9):733-8. doi: 10.1016/S2221-1691(12)60219-6.

Inhibition of α-amylase and α-glucosidase activities by ethanolic extract of Telfairia occidentalis (fluted pumpkin) leaf.

Author information

1
Department of Biochemistry,Federal University of Technology, Akure, NigeriaP.M.B., 704, Akure 340001, Nigeria.

Abstract

OBJECTIVE:

To investigate the inhibitory effect of Telfairia occidentalis Hook f. (Curcubitaceae) (T. occidentalis) leaf on key enzyme linked to type-2 diabetes (α - amylase and α - glucosidase) as well as assess the effect of blanching (a commonly practiced food processing technique) of the vegetable on these key enzymes.

METHODS:

Fresh leaves of T. occidentalis were blanched in hot water for 10 minutes, and the extracts of both the fresh and blanched vegetables were prepared and used for subsequent analysis. The inhibitory effect of the extract on α - amylase and α - glucosidase activities as well as some antioxidant parameter was determined in vitro.

RESULTS:

The result revealed that unprocessed T. occidentalis leaf reduce Fe(3+) to Fe(2+) and also inhibited α - amylase and α - glucosidase activities in a dose dependent manner. However, blanching of the leafy vegetables caused a significant (P<0.05) increase in the antioxidant properties but decrease their ability to inhibit α - amylase and α - glucosidase activities.

CONCLUSIONS:

This antioxidant properties and enzyme inhibition could be part of the mechanism by which they are used in the treatment/prevention of type-2 diabetes. However, the blanched vegetable reduces their ability to inhibit both α - amylase and α - glucosidase activity in vitro.

KEYWORDS:

Antioxidants; Blanching; Telfairia occidentalis; Vegetables; α – amylase; α – glucosidase

PMID:
23570004
PMCID:
PMC3609380
DOI:
10.1016/S2221-1691(12)60219-6
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science Icon for PubMed Central
    Loading ...
    Support Center