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Asian Pac J Trop Biomed. 2011 Jan;1(1):49-53. doi: 10.1016/S2221-1691(11)60067-1.

Assessment of in vitro sensitivity of Plasmodium vivax fresh isolates.

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1
Pharmacology and Toxicology Unit, Graduate Program in Biomedical Sciences, Thammasat University, Thailand.

Abstract

OBJECTIVE:

To compare the applicability of the SYBR Green-I assay with the standard schizont maturation assay, for determination of sensitivity of Plasmodium vivax (P. vivax) to chloroquine and a new antifolate WR 99210.

METHODS:

The study was conducted at Mae Tao Clinic for migrant workers, Tak Province during April 2009 to July 2010. A total of 64 blood samples (1 mL blood collected into sodium heparinized plastic tube) were collected from patients with mono-infection with P. vivax malaria prior to treatment with standard regimen of a 3-day chloroquine. In vitro sensitivity of P. vivax isolates was evaluated by schizont maturation inhibition and SYBR Green-I assays.

RESULTS:

A total of 30 out of 64 blood samples collected from patients with P. vivax malaria were successfully analyzed using both the microscopic schizont maturation inhibition and SYBR Green-I assays. The failure rates of the schizont maturation inhibition assay (50%) and the SYBR Green-I assay (54%) were similar (P=0.51). The median IC10s, IC50s and IC90s of both chloroquine and WR99210 were not significantly different from the clinical isolates of P. vivax tested. Based on the cut-off of 100 nM, the prevalences of chloroquine resistance determined by schizont maturation inhibition and SYBR Green-I assays were 19 and 11 isolates, respectively. The strength of agreement between the two methods was very poor for both chloroquine and WR99210.

CONCLUSIONS:

On the basis of this condition and its superior sensitivity, the microscopic method appears better than the SYBR Green-I Green assay for assessing in vitro sensitivity of fresh P. vivax isolates to antimalarial drugs.

KEYWORDS:

Antifolate; Antimalarial drug; Chloroquine; Fresh isolate; In vitro sensitivity; Malaria; Plasmodium vivax; SYBR Green-I assay; Schizont maturation inhibition assay; Standard regimen

PMID:
23569724
PMCID:
PMC3609160
DOI:
10.1016/S2221-1691(11)60067-1
[Indexed for MEDLINE]
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