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FASEB J. 2013 Jul;27(7):2799-806. doi: 10.1096/fj.13-229286. Epub 2013 Apr 8.

Chorein sensitivity of cytoskeletal organization and degranulation of platelets.

Author information

1
Department of Physiology, University of Tübingen, Gmelinstrasse 5, 72076 Tübingen, Germany.

Abstract

Chorea-acanthocytosis (ChAc), a lethal disease caused by defective chorein, is characterized by neurodegeneration and erythrocyte acanthocytosis. The functional significance of chorein in other cell types remained ill-defined. The present study revealed chorein expression in blood platelets. As compared to platelets from healthy volunteers, platelets from patients with ChAc displayed a 47% increased globular/filamentous actin ratio, indicating actin depolymerization. Moreover, phosphoinositide-3-kinase subunit p85 phosphorylation, p21 protein-activated kinase (PAK1) phosphorylation, as well as vesicle-associated membrane protein 8 (VAMP8) expression were significantly reduced in platelets from patients with ChAc (by 17, 22, and 39%, respectively) and in megakaryocytic (MEG-01) cells following chorein silencing (by 16, 54, and 11%, respectively). Activation-induced platelet secretion from dense granules (ATP release) and α granules (P-selectin exposure) were significantly less (by 55% after stimulation with 1 μg/ml CRP and by 33% after stimulation with 5 μM TRAP, respectively) in ChAc platelets than in control platelets. Furthermore, platelet aggregation following stimulation with different platelet agonists was significantly impaired. These observations reveal a completely novel function of chorein, i.e., regulation of secretion and aggregation of blood platelets.

KEYWORDS:

PI3K; VAMP8; aggregation; chorea-acanthocytosis

PMID:
23568775
DOI:
10.1096/fj.13-229286
[Indexed for MEDLINE]

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