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Anal Bioanal Chem. 2013 May;405(14):4969-73. doi: 10.1007/s00216-013-6888-z. Epub 2013 Apr 10.

Versatile synthesis of probes for high-throughput enzyme activity screening.

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1
Department of Chemistry, University of California, Berkeley, CA 94720, USA.

Abstract

Mass spectrometry based technologies are promising as generalizable high-throughput assays for enzymatic activity. In one such technology, a specialized enzyme substrate probe is presented to a biological mixture potentially exhibiting enzymatic activity, followed by an in situ enrichment step using fluorous interactions and nanostructure-initiator mass spectrometry. This technology, known as Nimzyme, shows great potential but is limited by the need to synthesize custom substrate analogs. We describe a synthetic route that simplifies the production of these probes by fashioning their perfluorinated invariant portion as an alkylating agent. This way, a wide variety of compounds can be effectively transformed into enzyme activity probes. As a proof of principle, a chloramphenicol analog synthesized according to this methodology was used to detect chloramphenicol acetyltransferase activity in cell lysate. This verifies the validity of the synthetic strategy employed and constitutes the first reported application of Nimzyme to a non-carbohydrate-active enzyme. The simplified synthetic approach presented here may help advance the application of mass spectrometry to high-throughput enzyme activity determination.

PMID:
23568610
PMCID:
PMC3637890
DOI:
10.1007/s00216-013-6888-z
[Indexed for MEDLINE]
Free PMC Article
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