A novel marker for the species-specific detection and quantitation of Vibrio cholerae by targeting an outer membrane lipoprotein lolB gene

J Microbiol Biotechnol. 2013 Apr;23(4):555-9. doi: 10.4014/jmb.1208.08029.

Abstract

Vibrio cholerae O1 and O139 are the major serotypes associated with illness, and some V. cholera non-O1 and non-O139 isolates produce cholera toxin. The present study describes a quantitative polymerase chain reaction (qPCR) assay for the species-specific detection and quantitation of V. cholera using a primer pair based on an outer membrane lipoprotein lolB gene for the amplification of a 195 bp DNA fragment. The qPCR primer set for the accurate diagnosis of V. cholera was developed from publically available genome sequences. This quantitative PCR-based method will potentially simplify and facilitate the diagnosis of this pathogen and guide disease management.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Load / methods*
  • Bacterial Outer Membrane Proteins / genetics*
  • Cholera / diagnosis
  • Cholera / microbiology
  • DNA Primers / genetics
  • Lipoproteins / genetics*
  • Real-Time Polymerase Chain Reaction / methods*
  • Vibrio cholerae O1 / genetics
  • Vibrio cholerae O1 / isolation & purification*

Substances

  • Bacterial Outer Membrane Proteins
  • DNA Primers
  • Lipoproteins