Format

Send to

Choose Destination
Biosci Biotechnol Biochem. 2013;77(4):776-81. Epub 2013 Apr 7.

Doxycycline-dependent inducible and reversible RNA interference mediated by a single lentivirus vector.

Author information

1
Functional Genomics Core Laboratory, Ehime University Proteo-Medicine Research Center (ProMRes), Ehime, Japan. natsukim@m.ehime-u.ac.jp

Abstract

RNA interference has been applied to the development of a method of silencing genes of interest. Short hairpin RNA (shRNA) expression vectors are useful in driving gene-silencing. Standard shRNA vectors produce a knockdown phenotype soon after transduction. An alternative strategy for conditional gene knockdown would be useful to investigate gene functions in a time-dependent manner. In this study, we developed an inducible gene knockdown system based on lentivirus-mediated gene transfer. A single lentivirus vector capable of inducible expression of a designed microRNA-based shRNA was generated using a tetracycline-dependent transactivation system. The lentiviral vector facilitated doxycycline-dependent inducible knockdown specific to the target gene. Withdrawal of doxycycline after transient treatment resulted in complete recovery of target gene expressions to normal levels. Thus the single lentiviral vector developed in this study should be a powerful tool for doxycycline-dependent inducible and reversible RNA interference in molecular genetic studies.

PMID:
23563548
DOI:
10.1271/bbb.120917
[Indexed for MEDLINE]
Free full text

Supplemental Content

Full text links

Icon for Taylor & Francis Icon for J-STAGE, Japan Science and Technology Information Aggregator, Electronic
Loading ...
Support Center