Format

Send to

Choose Destination
Anal Biochem. 2013 Jul 15;438(2):107-9. doi: 10.1016/j.ab.2013.03.026. Epub 2013 Apr 4.

Functional characterization of heat shock protein 90 targeted compounds.

Author information

1
Department of Pharmaceutical and Biological Chemistry, UCL School of Pharmacy, London WC1N 1AX, UK.

Abstract

Heat shock protein 90 (Hsp90) is an attractive cancer target that possesses two potential binding domains at the C and N termini. Nevertheless, assays that can reliably distinguish between the C- and N-terminal Hsp90 inhibitors and quantitatively characterize candidate drugs are limited. Here we report an Hsp90 binding assay and ATPase inhibition assay that can not only separate the C- and N-terminal inhibitors but also quantitatively determine their respective IC50 values.

PMID:
23563445
DOI:
10.1016/j.ab.2013.03.026
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center