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Br J Cancer. 2013 Apr 30;108(8):1712-9. doi: 10.1038/bjc.2013.121. Epub 2013 Apr 4.

Identification of serum microRNA profiles in colon cancer.

Author information

1
Department of Oncology, St Olavs Hospital, Trondheim University Hospital, Olav Kyrresgt 17, Trondheim 7006, Norway. eva.hofsli@stolav.no

Abstract

BACKGROUND:

microRNAs (miRNAs) exist in blood in an apparently stable form. We have explored whether serum miRNAs can be used as non-invasive early biomarkers of colon cancer.

METHODS:

Serum samples from 30 patients with colon cancer stage IV and 10 healthy controls were examined for the expression of 375 cancer-relevant miRNAs. Based on the miRNA profile in this study, 34 selected miRNAs were measured in serum from 40 patients with stage I-II colon cancer and from 10 additional controls.

RESULTS:

Twenty miRNAs were differentially expressed in serum from stage IV patients compared with controls (P<0.01). Unsupervised clustering revealed four subgroups; one corresponding mostly to the control group and the three others to the patient groups. Of the 34 miRNAs measured in the follow-up study of stage I-II patients, 21 showed concordant expression between stage IV and stage I-II patient. Based on the profiles of these 21 miRNAs, a supervised linear regression analysis (Partial Least Squares Regression) was performed. Using this model we correctly assigned stage I-II colon cancer patients based on miRNA profiles of stage IV patients.

CONCLUSION:

Serum miRNA expression profiling may be utilised in early detection of colon cancer.

PMID:
23558896
PMCID:
PMC3668463
DOI:
10.1038/bjc.2013.121
[Indexed for MEDLINE]
Free PMC Article
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