Format

Send to

Choose Destination
See comment in PubMed Commons below
J Tissue Eng Regen Med. 2015 Nov;9(11):E13-26. doi: 10.1002/term.1736. Epub 2013 Apr 1.

Agonism of Wnt-β-catenin signalling promotes mesenchymal stem cell (MSC) expansion.

Author information

1
Departments of Biomedical Engineering, Chemical Engineering and Orthopaedics and Center for Musculoskeletal Research, University of Rochester Medical Center, Rochester, NY, USA.

Abstract

Promoting mesenchymal stem cell (MSC) proliferation has numerous applications in stem cell therapies, particularly in the area of regenerative medicine. In order for cell-based regenerative approaches to be realized, MSC proliferation must be achieved in a controlled manner without compromising stem cell differentiation capacities. Here we demonstrate that 6-bromoindirubin-3'-oxime (BIO) increases MSC β-catenin activity 106-fold and stem cell-associated gene expression ~33-fold, respectively, over untreated controls. Subsequently, BIO treatment increases MSC populations 1.8-fold in typical 2D culture conditions, as well as 1.3-fold when encapsulated within hydrogels compared to untreated cells. Furthermore, we demonstrate that BIO treatment does not reduce MSC multipotency where MSCs maintain their ability to differentiate into osteoblasts, chondrocytes and adipocytes using standard conditions. Taken together, our results demonstrate BIO's potential utility as a proliferative agent for cell transplantation and tissue regeneration.

KEYWORDS:

6-bromoindirubin-3′-oxime; hydrogel biomaterials; mesenchymal stem cells; pluripotency; proliferation; regenerative medicine

PMID:
23554411
PMCID:
PMC4087086
DOI:
10.1002/term.1736
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Wiley Icon for PubMed Central
    Loading ...
    Support Center