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J Cell Biochem. 2013 Sep;114(9):2148-59. doi: 10.1002/jcb.24563.

MicroRNA-126 regulates EPCs function: implications for a role of miR-126 in preeclampsia.

Author information

1
Department of Gynecology and Obstetrics, Union Hospital, Huazhong University of Science and Technology, Wuhan, 430022, China.

Abstract

Preeclampsia is a specific vascular complication in pregnancy, which has a pathophysiology of altered endothelial homeostasis. There is extensive evidence that endothelial progenitor cells (EPCs) dysfunction underlies the endothelial cells loss that occurs during preeclampsia. MicroRNA-126 (miR-126), an angiogenesis-related miRNA, has been shown to have potential angiogenic effects both in cultured endothelial cells in vitro and ischemia-induced angiogenesis in vivo. However, whether miR-126 has therapeutic potential in placental vasculogenesis of preeclampsia remains unclear. In this report, we analyzed the EPCs number and expression of miR-126 in patients with preeclampsia, then investigated the effects of miR-126 on EPCs function and rat placenta by employing up-regulation and down-regulation strategies. We confirmed that miR-126 enhanced EPCs proliferation, differentiation and migration. However, a strong reduction in EPCs function was observed in vitro after miR-126 inhibitor transfection. MiR-126 exerts pro-angiogenic functions by suppressing the synthetize of antiangiogenic factors PIK3R2. Similar to miR-126 overexpression, PIK3R2 downregulation promoted EPCs function. In pregnant rats, we also found that miR-126 increased vascular sprouting, placenta and fetus weights. These findings suggest that miR-126 is essential for angiogenic properties of EPCs in vitro and placental vasculogenesis in vivo, providing basis for an alternative therapeutic approach in patients with preeclampsia.

KEYWORDS:

ENDOTHELIAL PROGENITOR CELLS; MICRORNA-126; PREECLAMPSIA; VASCULOGENESIS

PMID:
23553946
DOI:
10.1002/jcb.24563
[Indexed for MEDLINE]

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