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J Biomed Opt. 2013 Apr;18(4):046003. doi: 10.1117/1.JBO.18.4.046003.

Rapid quantification of pixel-wise fiber orientation data in micrographs.

Author information

1
Tufts University, Science & Technology Center, Department of Biomedical Engineering, 4 Colby Street, Medford, Massachusetts 02155, USA. Kyle.Quinn@tufts.edu

Abstract

Defining fiber orientation at each pixel within a medical image has traditionally been computationally intensive and prone to systematic errors. A weighted orientation vector summation algorithm capable of detecting fiber orientation simultaneously at each pixel within an image is presented. As a result, pixel-specific fiber orientation information with 2 deg to 3 deg accuracy can be determined within seconds, enabling the practical use of pixel-wise orientation data for characterizing structural anisotropy. This analysis technique has applicability and potential diagnostic utility for a variety of modalities, including second harmonic generation, scanning electron microscopy and immunohistochemical imaging is demonstrated.

PMID:
23552635
PMCID:
PMC3639785
DOI:
10.1117/1.JBO.18.4.046003
[Indexed for MEDLINE]
Free PMC Article

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