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Methods Mol Biol. 2013;997:57-72. doi: 10.1007/978-1-62703-348-0_6.

Generation of human-induced pluripotent stem cells (hiPSCs) using episomal vectors on defined Essential 8™ Medium conditions.

Author information

1
Primary and Stem Cell Systems, Life Technologies, Carlsbad, CA, USA.

Abstract

Human-induced pluripotent stem cells (iPSCs) are an important potential source of cells for regenerative medicine due to their inherent ability to differentiate into all cell types of the three germ layers. Generation of iPSCs with a non-integrating reprogramming method and in culture conditions that are completely absent of animal proteins will be ideal for such regenerative and cell therapy applications. Here we describe a method to generate non-integrating iPSCs using the Episomal iPSC Reprogramming Vectors.

PMID:
23546748
DOI:
10.1007/978-1-62703-348-0_6
[Indexed for MEDLINE]

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