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Lab Chip. 2013 Jul 21;13(14):2679-81. doi: 10.1039/c3lc50207d.

Highly sensitive fluorescent protein FRET detection using optofluidic lasers.

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1
Department of Biomedical Engineering, University of Michigan, 1101 Beal Ave., Ann Arbor, MI 48109, USA.

Abstract

We achieved optofluidic protein lasing using genetically encoded fluorescent protein FRET pairs linked by length-tunable peptides. Up to 25-fold reduction in the donor laser emission was observed when the donor and the acceptor were brought to close proximity, as compared to only 17% reduction in the donor emission using the conventional FRET detection. Our work opens a door to a broad range of applications in studying protein-protein interactions and protein-drug interactions.

PMID:
23545541
DOI:
10.1039/c3lc50207d
[Indexed for MEDLINE]
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