Format

Send to

Choose Destination
Carbohydr Polym. 2013 Apr 15;94(1):185-92. doi: 10.1016/j.carbpol.2012.12.070. Epub 2013 Jan 8.

Efficient gene transfection in the neurotypic cells by star-shaped polymer consisting of β-cyclodextrin core and poly(amidoamine) dendron arms.

Author information

1
Key Laboratory of Neurogenetics and Channelopathies of Guangdong Province and The Ministry of Education of China, Institute of Neuroscience and The Second Affiliated Hospital of Guangzhou Medical University, Guangzhou, 510260, Guangdong Province, China.

Abstract

In order to develop the effective vectors that had high gene transfection capability and low cytotoxicity in the neuronal cells, we tested the star-shaped polymer consisting of β-cyclodextrin core and poly(amidoamine) (PAMAM) dendron arms [β-CD-(D3)7] as the vector to transfect the human neuroblastoma SH-SY5Y cells. The physicochemical properties of the β-CD-(D3)7/plasmid DNA (pDNA) complexes were characterized by using gel electrophoresis, dynamic light scattering, transmission electron microscopy and zeta-potential experiments. Among the human neuroblastoma SH-SY5Y cells, β-CD-(D3)7/pDNA complex demonstrated a lower toxicity compared to those of PAMAM (G=4, with an ethylenediamine core)/pDNA complex. When the N/P ratio was over 20, it was observed that PAMAM had a faster increment in toxicity compared to β-CD-(D3)7. Fluorescent image, confocal microscopy image and flow cytometry showed that β-CD-(D3)7/pDNA complexes had significantly higher transgene activity than that of PAMAM/pDNA complexes. For example, the transfection efficiency was 20% and 7.5% for β-CD-(D3)7/pDNA and PAMAM/pDNA complexes, respectively. These results indicated that β-CD-(D3)7 might be a promising candidate for neurotypic cells gene delivery with the characteristics of good biocompatibility, relatively high gene transfection capability and potential in vivo gene delivery ability.

PMID:
23544527
DOI:
10.1016/j.carbpol.2012.12.070
[Indexed for MEDLINE]

Supplemental Content

Full text links

Icon for Elsevier Science
Loading ...
Support Center