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Cornea. 2013 Jun;32(6):847-50. doi: 10.1097/ICO.0b013e31828a27dd.

Evaluating DSAEK graft deturgescence in preservation medium after microkeratome cut with optical coherence tomography.

Author information

1
Center for Ophthalmic Optics & Lasers, Casey Eye Institute and Department of Ophthalmology, Oregon Health & Science University, Portland, OR, USA.

Abstract

PURPOSE:

To evaluate the Descemet stripping automated endothelial keratoplasty (DSAEK) graft deturgescence in preservation medium after microkeratome cut using Fourier domain optical coherence tomography.

METHODS:

The central and peripheral thickness of DSAEK grafts was measured by Fourier domain optical coherence tomography immediately after microkeratome cuts and 1, 2, 3, and 4 hours afterward. All measurements were taken when the grafts were stored in 4°C preservation medium. The hourly change in central graft thickness and graft shape (peripheral graft thicknes - central graft thickness) was calculated and tracked over time.

RESULTS:

Five DSAEK grafts were measured. The average central graft thickness was 188.7 ± 44.4 μm (range, 146-255 µm) immediately after microkeratome cuts. The average central graft thickness was 147.5 ± 33.0 μm (range, 116-190 µm) after 4 hours in preservation medium (P < 0.001). The average hourly change in central graft thickness was -30.5 μm (P = 0.0051), -8.6 μm (P = 0.055), -2.0 μm (P = 0.42), and 0.0 μm (P = 0.93) at 1, 2, 3, and 4 hours, respectively, after microkeratome cuts. The average hourly change in graft shape was insignificant.

CONCLUSIONS:

DSAEK grafts become thinner after microkeratome cut and stabilize at approximately 2 hours. Therefore, DSAEK graft thickness should be measured at 1.5 to 3 hours after microkeratome cut.

PMID:
23538636
PMCID:
PMC3651749
DOI:
10.1097/ICO.0b013e31828a27dd
[Indexed for MEDLINE]
Free PMC Article
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