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J Virol. 2013 Jun;87(11):6521-5. doi: 10.1128/JVI.00006-13. Epub 2013 Mar 27.

A novel PCR assay for quantification of HIV-1 RNA.

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1
Department of Medicine, Hopkins University School of Medicine, Baltimore, Maryland, USA.

Abstract

Current assays for quantification of HIV-1 virions rely on real-time reverse transcriptase (RT)-PCR detection of conserved regions of HIV-1 RNA and can be limited by detection of contaminating viral or plasmid DNA. We developed a novel RT-PCR assay using a reverse primer that hybridizes with the poly(A) tail of HIV-1 mRNAs, anchored by conserved viral nucleotides at the most distal region of the transcript. This assay can detect and quantify HIV-1 RNA with high specificity and sensitivity.

PMID:
23536672
PMCID:
PMC3648108
DOI:
10.1128/JVI.00006-13
[Indexed for MEDLINE]
Free PMC Article
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