Format

Send to

Choose Destination
J Nucl Med. 2013 Jun;54(6):969-76. doi: 10.2967/jnumed.112.109090. Epub 2013 Mar 27.

Molecular imaging of conscious, unrestrained mice with AwakeSPECT.

Author information

1
Oak Ridge National Laboratory, Oak Ridge, Tennessee, USA.

Abstract

We have developed a SPECT imaging system, AwakeSPECT, to enable molecular brain imaging of untrained mice that are conscious, unanesthetized, and unrestrained. We accomplished this with head tracking and motion correction techniques.

METHODS:

The capability of the system for motion-corrected imaging was demonstrated with a (99m)Tc-pertechnetate phantom, (99m)Tc-methylene diphosphonate bone imaging, and measurement of the binding potential of the dopamine transporter radioligand (123)I-ioflupane in mouse brain in the awake and anesthetized (isoflurane) states. Stress induced by imaging in the awake state was assessed through measurement of plasma corticosterone levels.

RESULTS:

AwakeSPECT provided high-resolution bone images reminiscent of those obtained from CT. The binding potential of (123)I-ioflupane in the awake state was on the order of 50% of that obtained with the animal under anesthesia, consistent with previous studies in nonhuman primates. Levels of stress induced were on the order of those seen in other behavioral tasks and imaging studies of awake animals.

CONCLUSION:

These results demonstrate the feasibility of SPECT molecular brain imaging of mice in the conscious, unrestrained state and demonstrate the effects of isoflurane anesthesia on radiotracer uptake.

KEYWORDS:

SPECT; anesthesia; awake; ioflupane; mouse

PMID:
23536223
PMCID:
PMC3965297
DOI:
10.2967/jnumed.112.109090
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center