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Chem Commun (Camb). 2013 May 8;49(36):3754-6. doi: 10.1039/c3cc00070b. Epub 2013 Mar 27.

Quantitative detection of single base mutation by combining PNA hybridization and MALDI-TOF mass analysis.

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1
R&D Center, Diatech Korea Co. Ltd., Seoul 138-200, Republic of Korea.

Abstract

Peptide nucleic acid (PNA) probes were designed to bind to the internal reference sequence and the single base mutation sequence within PCR-amplified DNA templates. PNAs hybridized to the target sequences on DNA were analyzed using MALDI-TOF mass spectrometry. Accurate quantification of the relative amount of mutant DNA was reproducibly demonstrated.

PMID:
23536008
DOI:
10.1039/c3cc00070b
[Indexed for MEDLINE]
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