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Sci Rep. 2013;3:1569. doi: 10.1038/srep01569.

Long-term strain improvements accumulate mutations in regulatory elements responsible for hyper-production of cellulolytic enzymes.

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1
State Key Laboratory of Microbial Technology, Shandong University, Jinan, Shandong, China.

Abstract

Long-term strain improvements through repeated mutagenesis and screening have generated a hyper-producer of cellulases and hemicellulases from Penicillium decumbens 114 which was isolated 30 years ago. Here, the genome of the hyper-producer P. decumbens JU-A10-T was sequenced and compared with that of the wild-type strain 114-2. Further, the transcriptomes and secretomes were compared between the strains. Selective hyper-production of cellulases and hemicellulases but not all the secreted proteins was observed in the mutant, making it a more specific producer of lignocellulolytic enzymes. Functional analysis identified that changes in several transcriptional regulatory elements played crucial roles in the cellulase hyper-producing characteristics of the mutant. Additionally, the mutant showed enhanced supply of amino acids and decreased synthesis of secondary metabolites compared with the wild-type. The results clearly point out that we can target gene regulators and promoters with minimal alterations of the genetic content but maximal effects in genetic engineering.

PMID:
23535838
PMCID:
PMC3610096
DOI:
10.1038/srep01569
[Indexed for MEDLINE]
Free PMC Article
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