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Chem Commun (Camb). 2013 May 8;49(36):3766-8. doi: 10.1039/c3cc40454d. Epub 2013 Mar 28.

Functional assembly of a multi-enzyme methanol oxidation cascade on a surface-displayed trifunctional scaffold for enhanced NADH production.

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1
Department of Chemical and Biomolecular Engineering, University of Delaware, Newark, DE 19716, USA.

Abstract

We report a simple and low-cost strategy that allows the sequential and site-specific assembly of a dehydrogenase-based multi-enzyme cascade for methanol oxidation on the yeast surface using the high-affinity interactions between three orthogonal cohesin-dockerin pairs. The multi-enzyme cascade showed 5 times higher NADH production rate than the non-complexed enzyme mixture, a result of efficient substrate channeling.

PMID:
23535691
DOI:
10.1039/c3cc40454d
[Indexed for MEDLINE]
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