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Bioanalysis. 2013 Apr;5(7):819-26. doi: 10.4155/bio.13.42.

Automated liquid microjunction surface sampling-HPLC-MS/MS analysis of drugs and metabolites in whole-body thin tissue sections.

Author information

1
Organic & Biological Mass Spectrometry Group, Chemical Sciences Division, Oak Ridge National Laboratory, Oak Ridge, TN 37831-6131, USA. kerteszv@ornl.gov

Abstract

BACKGROUND:

The aim of this work was to develop a fully automated liquid extraction-based surface sampling system utilizing a commercially available autosampler coupled with HPLC-MS/MS detection.

RESULTS:

Discrete spots selected for droplet-based sampling and automated sample queue generation, for both the autosampler and MS, were enabled by using in-house developed software. In addition, co-registration of spatially resolved sampling positions and HPLC-MS information to generate heat maps of compounds monitored for subsequent data analysis was also available in the software. The system was evaluated with whole-body thin tissue sections from propranolol-dosed rats.

CONCLUSION:

The spatial distributions of both the drug and its hydroxypropranolol glucuronide metabolites were consistent with previous studies employing other liquid extraction-based surface sampling methodologies.

PMID:
23534426
DOI:
10.4155/bio.13.42
[Indexed for MEDLINE]

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