Format

Send to

Choose Destination
See comment in PubMed Commons below
Bacteriophage. 2012 Oct 1;2(4):239-255.

Dualities in the analysis of phage DNA packaging motors.

Author information

1
Department of Biochemistry; The University of Texas Health Science Center; San Antonio, TX USA.

Abstract

The DNA packaging motors of double-stranded DNA phages are models for analysis of all multi-molecular motors and for analysis of several fundamental aspects of biology, including early evolution, relationship of in vivo to in vitro biochemistry and targets for anti-virals. Work on phage DNA packaging motors both has produced and is producing dualities in the interpretation of data obtained by use of both traditional techniques and the more recently developed procedures of single-molecule analysis. The dualities include (1) reductive vs. accretive evolution, (2) rotation vs. stasis of sub-assemblies of the motor, (3) thermal ratcheting vs. power stroking in generating force, (4) complete motor vs. spark plug role for the packaging ATPase, (5) use of previously isolated vs. new intermediates for analysis of the intermediate states of the motor and (6) a motor with one cycle vs. a motor with two cycles. We provide background for these dualities, some of which are under-emphasized in the literature. We suggest directions for future research.

KEYWORDS:

ATPase; DNA packaging; bacteriophage genetics; bacteriophage structure; biological motor; cryo-electron microscopy; single-molecule analysis

PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Taylor & Francis Icon for PubMed Central
    Loading ...
    Support Center