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Nan Fang Yi Ke Da Xue Xue Bao. 2013 Mar;33(3):318-21.

Promoter methylation of Wilms' tumor gene on the X- chromosome in gastric cancer.

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1
Department of Pathology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China. a200550346@yahoo.com.cn

Abstract

OBJECTIVE:

To investigate the changes in methylation levels of the promoters of the tumor suppressor gene Wilms' tumor gene on the X-chromosome (WTX) and its possible role in gastric cancer.

METHODS:

WTX promoter methylation levels were detected in 20 pairs of specimens of gastric cancer and matched normal tissues and in 3 gastric cancer cell lines (MGC803, SCG7901, and BGC823) using the Sequenom MassARRAY quantitative analysis system. The gastric cancer cell line BGC823 was treated with 5-aza-2'-deoxycytidine (5-aza-dC) for demethylation and the changes in the level of WTX promoter methylation were investigated.

RESULTS:

WTX promoter methylation levels were very low and showed no significant differences among normal gastric tissues, gastric cancer tissues and the 3 gastric cancer cell lines. In BGC823 cells, treatment with 5-aza-dC did not obviously affect the promoter methylation levels of WTX.

CONCLUSION:

High methylation levels of WTX promoters are rare in gastric cancer.

PMID:
23529223
[Indexed for MEDLINE]
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