Analysis of impurities in vertilmicin sulfate by liquid chromatography ion-trap mass spectrometry

J Pharm Biomed Anal. 2013 Jun:80:1-8. doi: 10.1016/j.jpba.2013.02.026. Epub 2013 Feb 27.

Abstract

The characterization of impurities present in vertilmicin by liquid chromatography (LC) coupled with mass spectrometry (MS) is described. A reversed phase (RP)-LC method using a C18 column resistant to basic pH with an alkaline (pH 11) aqueous mobile phase was developed and coupled to MS with an electrospray ionization (ESI) source in the positive ion mode which provides MS(n) capability. In total, 18 impurities were detected in a commercial sample. Eleven impurities described in this work were newly identified.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aminoglycosides / analysis*
  • Aminoglycosides / chemistry
  • Anti-Bacterial Agents / analysis*
  • Anti-Bacterial Agents / chemistry
  • Chromatography, Liquid / methods*
  • Drug Contamination
  • Hydrogen-Ion Concentration
  • Spectrometry, Mass, Electrospray Ionization / methods

Substances

  • Aminoglycosides
  • Anti-Bacterial Agents
  • vertilmicin