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EMBO J. 2013 Apr 17;32(8):1141-54. doi: 10.1038/emboj.2013.56. Epub 2013 Mar 19.

Human microcephaly protein CEP135 binds to hSAS-6 and CPAP, and is required for centriole assembly.

Author information

1
Institute of Biomedical Sciences, Academia Sinica, Taipei 11529, Taiwan, ROC.

Abstract

Centrioles are cylindrical structures that are usually composed of nine triplets of microtubules (MTs) organized around a cartwheel-shaped structure. Recent studies have proposed a structural model of the SAS-6-based cartwheel, yet we do not know the molecular detail of how the cartwheel participates in centriolar MT assembly. In this study, we demonstrate that the human microcephaly protein, CEP135, directly interacts with hSAS-6 via its carboxyl-terminus and with MTs via its amino-terminus. Unexpectedly, CEP135 also interacts with another microcephaly protein CPAP via its amino terminal domain. Depletion of CEP135 not only perturbed the centriolar localization of CPAP, but also blocked CPAP-induced centriole elongation. Furthermore, CEP135 depletion led to abnormal centriole structures with altered numbers of MT triplets and shorter centrioles. Overexpression of a CEP135 mutant lacking the proper interaction with hSAS-6 had a dominant-negative effect on centriole assembly. We propose that CEP135 may serve as a linker protein that directly connects the central hub protein, hSAS-6, to the outer MTs, and suggest that this interaction stabilizes the proper cartwheel structure for further CPAP-mediated centriole elongation.

PMID:
23511974
PMCID:
PMC3630357
DOI:
10.1038/emboj.2013.56
[Indexed for MEDLINE]
Free PMC Article

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