Format

Send to

Choose Destination
Antimicrob Agents Chemother. 2013 Jun;57(6):2838-40. doi: 10.1128/AAC.00056-13. Epub 2013 Mar 18.

Simple resazurin-based microplate assay for measuring Chlamydia infections.

Author information

1
Department of Molecular Biosciences, University of Kansas, Lawrence, Kansas, USA.

Abstract

The conventional method for quantification of Chlamydia infection using fluorescence microscopy typically involves time- and labor-intensive manual enumeration, which is not applicable for a large-scale analysis required for an inhibitory compound screen. In this study, an alamarBlue (resazurin) assay was adopted to measure Chlamydia infection by measuring the redox capability of infected host cells in a 96-well format. The assay provided measurements comparable to those of the conventional microscopy method while drastically reducing the time required for analysis.

PMID:
23507273
PMCID:
PMC3716173
DOI:
10.1128/AAC.00056-13
[Indexed for MEDLINE]
Free PMC Article

Supplemental Content

Full text links

Icon for HighWire Icon for PubMed Central
Loading ...
Support Center