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J Neurochem. 2013 Jul;126(2):203-12. doi: 10.1111/jnc.12243. Epub 2013 Apr 23.

STED microscopy of living cells--new frontiers in membrane and neurobiology.

Author information

1
Department of Nanobiophotonics, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany. ceggeli@gwdg.de

Abstract

Recent developments in fluorescence far-field microscopy such as STED microscopy have accomplished observation of the living cell with a spatial resolution far below the diffraction limit. Here, we briefly review the current approaches to super-resolution optical microscopy and present the implementation of STED microscopy for novel insights into live cell mechanisms, with a focus on neurobiology and plasma membrane dynamics.

KEYWORDS:

STED nanoscopy; fluorescence imaging; membrane dynamics

PMID:
23506404
DOI:
10.1111/jnc.12243
[Indexed for MEDLINE]
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