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Synapse. 2013 Oct;67(10):648-55. doi: 10.1002/syn.21666. Epub 2013 Apr 18.

Hippocampal serotonin-1A receptor function in a mouse model of anxiety induced by long-term voluntary wheel running.

Author information

1
RG Animal Models in Psychiatry, Department of Psychiatry and Psychotherapy, Central Institute of Mental Health, Medical Faculty Mannheim, Heidelberg University, J 5, 68159 Mannheim, Germany.
2
Department of Radiooncology, Medical Faculty Heidelberg, Heidelberg University, Im Neuenheimer Feld 400, 69120 Heidelberg, Germany.
3
Department of Pharmacology, University of Texas Health Science Center-San Antonio, 7703 Floyd Curl Drive, San Antonio, TX 78229-3900, United States.
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Contributed equally

Abstract

We have recently demonstrated that, in C57/Bl6 mice, long-term voluntary wheel running is anxiogenic, and focal hippocampal irradiation prevents the increase in anxiety-like behaviors and neurobiological changes in the hippocampus induced by wheel running. Evidence supports a role of hippocampal 5-HT1A receptors in anxiety. Therefore, we investigated hippocampal binding and function of 5-HT1A receptors in this mouse model of anxiety. Four weeks of voluntary wheel running resulted in hippocampal subregion-specific changes in 5-HT1A receptor binding sites and function, as measured by autoradiography of [(3) H] 8-hydroxy-2-(di-n-propylamino)tetralin binding and agonist-stimulated binding of [(35) S]GTPγS to G proteins, respectively. In the dorsal CA1 region, 5-HT1A receptor binding and function were not altered by wheel running or irradiation. In the dorsal dentate gyrus and CA2/3 region, 5-HT1A receptor function was decreased by not only running but also irradiation. In the ventral pyramidal layer, wheel running resulted in a decrease of 5-HT1A receptor function, which was prevented by irradiation. Neither irradiation nor wheel running affected 5-HT1A receptors in medial prefrontal cortex or in the dorsal or median raphe nuclei. Our data indicate that downregulation of 5-HT1A receptor function in ventral pyramidal layer may play a role in anxiety-like behavior induced by wheel running.

KEYWORDS:

exercise; hippocampus; neurogenesis; raphe nucleus; serotonin

PMID:
23505009
PMCID:
PMC3816532
DOI:
10.1002/syn.21666
[Indexed for MEDLINE]
Free PMC Article
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