Format

Send to

Choose Destination
See comment in PubMed Commons below
Nat Methods. 2013 May;10(5):403-6. doi: 10.1038/nmeth.2407. Epub 2013 Mar 17.

Barcoding cells using cell-surface programmable DNA-binding domains.

Author information

1
Department of Genetics, Harvard Medical School, Boston, Massachusetts, USA.

Abstract

We report an approach to barcode cells through cell-surface expression of programmable zinc-finger DNA-binding domains (surface zinc fingers, sZFs). We show that sZFs enable sequence-specific labeling of living cells by dsDNA, and we develop a sequential labeling approach to image more than three cell types in mixed populations using three fluorophores. We demonstrate the versatility of sZFs through applications in which they serve as surrogate reporters, function as selective cell capture reagents and facilitate targeted cellular delivery of viruses.

PMID:
23503053
PMCID:
PMC3641172
DOI:
10.1038/nmeth.2407
[Indexed for MEDLINE]
Free PMC Article
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Nature Publishing Group Icon for PubMed Central
    Loading ...
    Support Center