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Mol Cell Endocrinol. 2013 Jun 15;372(1-2):30-41. doi: 10.1016/j.mce.2013.02.019. Epub 2013 Mar 14.

Juvenile hormone and insulin suppress lipolysis between periods of lactation during tsetse fly pregnancy.

Author information

1
Janelia Farm Research Campus, Howard Hughes Medical Institute, Ashburn, VA.
2
Department of Epidemiology of Microbial Diseases, School of Public Health, Yale University, New Haven, CT 06520.
3
Section of Molecular and Applied Zoology, Institute of Zoology, Slovak Academy of Sciences, Bratislava, Slovakia.
4
Department of Biochemistry and Molecular Biology, Egerton University, Njoro, Kenya.
5
Department of Entomology and Plant Pathology, University of Tennessee, Knoxville TN.
6
Department of Evolution, Ecology, and Organismal Biology, Ohio State University, Columbus, OH.
#
Contributed equally

Abstract

Tsetse flies are viviparous insects that nurture a single intrauterine progeny per gonotrophic cycle. The developing larva is nourished by the lipid-rich, milk-like secretions from a modified female accessory gland (milk gland). An essential feature of the lactation process involves lipid mobilization for incorporation into the milk. In this study, we examined roles for juvenile hormone (JH) and insulin/IGF-like (IIS) signaling pathways during tsetse pregnancy. In particular, we examined the roles for these pathways in regulating lipid homeostasis during transitions between non-lactating (dry) and lactating periods. The dry period occurs over the course of oogenesis and embryogenesis, while the lactation period spans intrauterine larvigenesis. Genes involved in the JH and IIS pathways were upregulated during dry periods, correlating with lipid accumulation between bouts of lactation. RNAi suppression of Forkhead Box Sub Group O (FOXO) expression impaired lipolysis during tsetse lactation and reduced fecundity. Similar reduction of the JH receptor Methoprene tolerant (Met), but not its paralog germ cell expressed (gce), reduced lipid accumulation during dry periods, indicating functional divergence between Met and gce during tsetse reproduction. Reduced lipid levels following Met knockdown led to impaired fecundity due to inadequate fat reserves at the initiation of milk production. Both the application of the JH analog (JHA) methoprene and injection of insulin into lactating females increased stored lipids by suppressing lipolysis and reduced transcripts of lactation-specific genes, leading to elevated rates of larval abortion. To our knowledge, this study is the first to address the molecular physiology of JH and IIS in a viviparous insect, and specifically to provide a role for JH signaling through Met in the regulation of lipid metabolism during insect lactation.

PMID:
23499946
PMCID:
PMC4222070
DOI:
10.1016/j.mce.2013.02.019
[Indexed for MEDLINE]
Free PMC Article

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