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Neuron. 2013 Apr 10;78(1):65-80. doi: 10.1016/j.neuron.2013.02.029. Epub 2013 Mar 14.

VCP is essential for mitochondrial quality control by PINK1/Parkin and this function is impaired by VCP mutations.

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1
Department of Developmental Neurobiology, St. Jude Children's Research Hospital, Memphis, TN 38120, USA.

Erratum in

  • Neuron. 2013 Apr 24;78(2):403.

Abstract

Mutations in VCP cause multisystem degeneration impacting the nervous system, muscle, and/or bone. Patients may present with ALS, Parkinsonism, frontotemporal dementia, myopathy, Paget's disease, or a combination of these. The disease mechanism is unknown. We developed a Drosophila model of VCP mutation-dependent degeneration. The phenotype is reminiscent of PINK1 and parkin mutants, including a pronounced mitochondrial defect. Indeed, VCP interacts genetically with the PINK1/parkin pathway in vivo. Paradoxically, VCP complements PINK1 deficiency but not parkin deficiency. The basis of this paradox is resolved by mechanistic studies in vitro showing that VCP recruitment to damaged mitochondria requires Parkin-mediated ubiquitination of mitochondrial targets. VCP recruitment coincides temporally with mitochondrial fission, and VCP is required for proteasome-dependent degradation of Mitofusins in vitro and in vivo. Further, VCP and its adaptor Npl4/Ufd1 are required for clearance of damaged mitochondria via the PINK1/Parkin pathway, and this is impaired by pathogenic mutations in VCP.

PMID:
23498974
PMCID:
PMC3683300
DOI:
10.1016/j.neuron.2013.02.029
[Indexed for MEDLINE]
Free PMC Article

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