Format

Send to

Choose Destination
See comment in PubMed Commons below
Expert Opin Med Diagn. 2008 Mar;2(3):263-75. doi: 10.1517/17530059.2.3.263.

Mitochondrial genome analysis in biofluids for early cancer detection and monitoring.

Author information

1
Senior Scientist Genesis Genomics, Inc., 290 Munro Street, Ste 1000, Thunder Bay, Ontario, P7A 7T1, Canada +1 807 768 4516 ; +1 807 346 8105 ; gabriel.dakubo@genesisgenomics.com.

Abstract

BACKGROUND:

Biofluids collected in a non-invasive fashion are potentially valuable samples for assaying genomic alterations for early detection and monitoring of cancer. The low cellularity and nucleic acid content in biofluids, the high copy number of the mitochondrial genome (mtgenome) and its noted early imprints in cancer make this molecule theoretically more sensitive than nuclear targets to measure for early cancer detection.

OBJECTIVE:

This review explores mtgenome analysis in biofluids and addresses the question of whether targeting the mtgenome in biofluids is superior or equivalent to analysis of nuclear genomic alterations.

METHODS:

The literature was retrieved from PubMed using a combination of the following keywords: mtDNA, mutation, deletion, content, copy number, cancer, biofluids, bodily fluids and the specific cancers described here. Studies that analyzed mtgenome alterations in biofluids were included. Analytical methods available for assaying mtgenome changes in biofluids are discussed.

RESULTS:

Despite the limited data available, mtgenome changes in biofluids have been demonstrated in a wide variety of cancer patients.

CONCLUSION:

Mtgenome analysis in biofluids is feasible and relatively easy. Despite the paucity of data, tumor-specific mtgenome changes are observed in biofluids of cancer patients. Given the multiple copies per cell of the mtgenome, future cancer detection efforts should consider complementary analysis of mtgenome changes in biofluids.

PMID:
23495657
DOI:
10.1517/17530059.2.3.263
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Taylor & Francis
    Loading ...
    Support Center