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Adv Healthc Mater. 2013 Sep;2(9):1224-8. doi: 10.1002/adhm.201200380. Epub 2013 Mar 12.

μHall chip for sensitive detection of bacteria.

Author information

1
Center for Systems Biology, Massachusetts General Hospital, 185 Cambridge St, CPZN 5206, Boston, MA 02114.

Abstract

Sensitive, rapid and phenotype-specific enumeration of pathogens is essential for the diagnosis of infectious disease, monitoring of food chains, and for defense against bioterrorism. Microbiological culture and genotyping, techniques that sensitively and selectively detect bacteria in laboratory settings, have limited application in clinical environments due to high cost, slow response times, and the need for specially trained staff and laboratory infrastructure. To address these challenges, we developed a microfluidic chip-based micro-Hall (μHall) platform capable of measuring single, magnetically tagged bacteria directly in clinical specimens with minimal sample processing. We demonstrated the clinical utility of the μHall chip by enumerating Gram-positive bacteria. The overall detection limit of the system was similar to that of culture tests (~10 bacteria), but the assay time was 50-times faster. This low-cost, single-cell analytical technique is especially well-suited to diagnose infectious diseases in resource-limited clinical settings.

KEYWORDS:

bacterial detection; magnetic nanoparticles; μHall

PMID:
23495188
PMCID:
PMC4340655
DOI:
10.1002/adhm.201200380
[Indexed for MEDLINE]
Free PMC Article

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