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Methods Mol Biol. 2013;983:125-38. doi: 10.1007/978-1-62703-302-2_7.

Identification and verification of microRNAs by high-throughput sequencing.

Author information

1
School of Biotechnology, Division of Gene Technology, Science for Life Laboratory (SciLifeLab Stockholm), KTH Royal Institute of Technology, Solna, Sweden.

Abstract

High-throughput sequencing methods have become invaluable for detection and analysis of small RNAs. The results are millions of sequences that need to be carefully analyzed by computational methods and preferentially verified by different experimental techniques. Here we describe how to use high-throughput sequencing followed by bioinformatics and northern blot to identify one particular class of small RNA, microRNAs.

PMID:
23494305
DOI:
10.1007/978-1-62703-302-2_7
[Indexed for MEDLINE]

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