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Microbiol Immunol. 2013 Mar;57(3):246-51. doi: 10.1111/1348-0421.12029.

Simple method for differentiating measles vaccine from wild-type strains using loop-mediated isothermal amplification.

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Laboratory of Virus Infection, Kitasato Institute for Life Sciences, Shirokane 5-9-1, Minato-ku, Tokyo, 108-8641, Japan. tetsuo‐n@lisci.kitasato‐


Because of increasing measles vaccine coverage, the proportion of patients with modified measles has been increasing. Such patients have low-grade fever with very mild eruptions similar to vaccine-related adverse events. Differentiation between these two pathogenic conditions is required to improve the quality of laboratory-based measles surveillance. In this study, vaccine-specific and wild-type specific primer sets were designed for loop-mediated isothermal amplification in the N gene, and vaccine strains, C1, D3, D4, D5, D8, D9, G3 and H1 wild strains were examined. Three vaccine strains were efficiently amplified using a vaccine-specific primer set with an approximately 10-times higher sensitivity than wild-type primer. Modified measles was differentiated from vaccine-associated cases by this system, but limitations were encountered with the other genotypes.

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