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J Am Chem Soc. 2013 Apr 3;135(13):4906-9. doi: 10.1021/ja311960g. Epub 2013 Mar 21.

RNA-based fluorescent biosensors for live cell imaging of second messengers cyclic di-GMP and cyclic AMP-GMP.

Author information

1
Department of Chemistry and ‡Department of Molecular & Cell Biology, University of California , Berkeley, California 94720, United States.

Abstract

Cyclic dinucleotides are an important class of signaling molecules that regulate a wide variety of pathogenic responses in bacteria, but tools for monitoring their regulation in vivo are lacking. We have designed RNA-based fluorescent biosensors for cyclic di-GMP and cyclic AMP-GMP by fusing the Spinach aptamer to variants of a natural GEMM-I riboswitch. In live cell imaging experiments, these biosensors demonstrate fluorescence turn-on in response to cyclic dinucleotides, and they were used to confirm in vivo production of cyclic AMP-GMP by the enzyme DncV.

PMID:
23488798
PMCID:
PMC3775879
DOI:
10.1021/ja311960g
[Indexed for MEDLINE]
Free PMC Article

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