Format

Send to

Choose Destination
See comment in PubMed Commons below
Vet Parasitol. 2013 May 20;194(2-4):222-5. doi: 10.1016/j.vetpar.2013.02.001. Epub 2013 Feb 9.

Screening of early antigen genes of adult-stage Trichinella spiralis using pig serum from different stages of early infection.

Author information

1
Key Laboratory for Zoonosis Research, Ministry of Education, Jilin University, Zoonosis Center of National Institute of Parasitic Diseases, Chinese Center for Disease Control and Prevention, China.

Abstract

The goal of this work was to identify novel, early antigens present in Trichinella spiralis. To this end, a cDNA library generated from 3-day old adult worms (Ad3) was immunologically screened using serum from a pig infected with 20,000 muscle larvae. The serum was obtained from multiple, time course bleeds coinciding with early worm development. Seventeen positive clones were isolated using serum obtained at 20 days post infection (dpi). All clones corresponded to one gene that exhibited high sequence identity with the T. spiralis ATP-dependent RNA helicase DDX19B which is involved in parasite growth and development. In addition, nine additional positive clones representing 5 unique genes were identified when the library was screened with 30 dpi serum; four of these five genes displayed high similarity with members of a putative T. spiralis serine protease family known to be involved in host invasion and host-parasite interactions. The remaining gene aligned with the T. spiralis hypothetical ORF 11.30. The identification of these antigens provides potential candidates for the early diagnosis of trichinellosis and for the development of a vaccine against this parasite.

PMID:
23485437
DOI:
10.1016/j.vetpar.2013.02.001
[Indexed for MEDLINE]
PubMed Commons home

PubMed Commons

0 comments
How to join PubMed Commons

    Supplemental Content

    Full text links

    Icon for Elsevier Science
    Loading ...
    Support Center