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Int J Radiat Biol. 2013 Oct;89(10):832-40. doi: 10.3109/09553002.2013.784427. Epub 2013 Apr 16.

Effect of X-ray irradiation on expression of organic anion transporting polypeptides.

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1
College of Life Science, South China Agricultural University , Guangzhou, Guangdong Province.

Abstract

PURPOSE:

Some members of the organic anion transporting polypeptide (OATP) family may play critical roles in determining the concentration of anticancer drugs in targeted organs/cells. The purpose of the present study was to investigate whether radiation can alter the expression of OATP in cancer cells so that OATP can be used as a specific drug target for anti-tumor treatment in combination with radiotherapy.

MATERIALS AND METHODS:

Two cancer cell lines (HepG2 and MCF7) were irradiated with three doses of X-ray and the expression levels of six OATP were assessed.

RESULTS:

Expressions of several OATP were altered after irradiation. OATP1A2, an OATP that was found to transport anti-tumor drugs and was undetectable in untreated cells, showed dramatically elevated level of expression after irradiation in MCF7 cells. The combined treatment of X-rays and the OATP1A2 substrate methotrexate exerted a more significant effect on growth rate and cell death compared with radiation or methotrexate treatment alone. At certain time points, X-rays increased OATP1A2 level while suppressing the expression of efflux transporters.

CONCLUSIONS:

Radiation may simultaneously increase the expression of uptake transporters and decrease the level of efflux transporters, suggesting OATP may be considered a novel target for delivery of anti-neoplastic agents in combination with radiotherapy.

PMID:
23484949
DOI:
10.3109/09553002.2013.784427
[Indexed for MEDLINE]
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