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Dev Dyn. 2013 Jun;242(6):622-37. doi: 10.1002/dvdy.23961. Epub 2013 Apr 23.

Multiparametric image analysis of lung-branching morphogenesis.

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1
Howard Hughes Medical Institute, Department of Pediatrics, University of Colorado School of Medicine and Childrens's Hospital Colorado, Aurora, Colorado, USA.

Abstract

BACKGROUND:

Lung-branching morphogenesis is a fundamental developmental process, yet the cellular dynamics that occur during lung development and the molecular mechanisms underlying recent postulated branching modes are poorly understood.

RESULTS:

Here, we implemented a time-lapse video microscopy method to study the cellular behavior and molecular mechanisms of planar bifurcation and domain branching in lung explant- and organotypic cultures. Our analysis revealed morphologically distinct stages that are shaped at least in part by a combination of localized and orientated cell divisions and by local mechanical forces. We also identified myosin light-chain kinase as an important regulator of bud bifurcation, but not domain branching in lung explants.

CONCLUSIONS:

This live imaging approach provides a method to study cellular behavior during lung-branching morphogenesis and suggests the importance of a mechanism primarily based on oriented cell proliferation and mechanical forces in forming and shaping the developing lung airways.

PMID:
23483685
PMCID:
PMC3833092
DOI:
10.1002/dvdy.23961
[Indexed for MEDLINE]
Free PMC Article
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