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Trends Plant Sci. 2013 Apr;18(4):175-9. doi: 10.1016/j.tplants.2013.02.001. Epub 2013 Mar 5.

RNA-Seq: revelation of the messengers.

Author information

1
Plant-Microbe Interactions, Department of Biology, Utrecht University, Padualaan 8, 3584 CH Utrecht, The Netherlands.

Abstract

Next-generation RNA-sequencing (RNA-Seq) is rapidly outcompeting microarrays as the technology of choice for whole-transcriptome studies. However, the bioinformatics skills required for RNA-Seq data analysis often pose a significant hurdle for many biologists. Here, we put forward the concepts and considerations that are critical for RNA-Seq data analysis and provide a generic tutorial with example data that outlines the whole pipeline from next-generation sequencing output to quantification of differential gene expression.

PMID:
23481128
DOI:
10.1016/j.tplants.2013.02.001
[Indexed for MEDLINE]

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