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Cell Rep. 2013 Mar 28;3(3):638-45. doi: 10.1016/j.celrep.2013.02.007. Epub 2013 Mar 7.

Replication fork stability is essential for the maintenance of centromere integrity in the absence of heterochromatin.

Author information

1
Molecular & Computational Biology Program, University of Southern California, Los Angeles, CA 90089-2910, USA.

Abstract

The centromere of many eukaryotes contains highly repetitive sequences marked by methylation of histone H3K9 by Clr4(KMT1). This recruits multiple heterochromatin proteins, including Swi6 and Chp1, to form a rigid centromere and ensure accurate chromosome segregation. In the absence of heterochromatin, cells show an increased rate of recombination in the centromere, as well as chromosome loss. These defects are severely aggravated by loss of replication fork stability. Thus, heterochromatin proteins and replication fork protection mechanisms work in concert to prevent abnormal recombination, preserve centromere integrity, and ensure faithful chromosome segregation.

PMID:
23478021
PMCID:
PMC3652564
DOI:
10.1016/j.celrep.2013.02.007
[Indexed for MEDLINE]
Free PMC Article

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